石决明散加减方治疗肝经积热型前葡萄膜炎的临床研究

目的观察中药石决明散加减方治疗肝经积热型前葡萄膜炎的临床效果。方法前葡萄膜炎患者60例随机分为两组各30例,对照组采用眼局部治疗,治疗组在对照组的基础上加用中药石决明散加减方中医辨证治疗,对比分析两组治疗效果。结果总有效率:治疗组为86.7%,对照组为76.7%,两组比较差异有统计学意义(P0.05);两组在随访中,复发率治疗组为10.0%,对照组为26.7%,差异有统计学意义(P0.05);中西医结合治疗其疗效优于对照组。结论中药石决明散加减方配合眼局部治疗可以缩短病程,提高疗效,减少复发。     

健脾益肠散对溃疡性结肠炎大鼠结肠组织HSP70表达的影响

目的:探讨健脾益肠散对溃疡性结肠炎(UC)大鼠结肠组织热休克蛋白70(HSP70)蛋白和mRNA表达的影响。方法:将健康SPF级雄性SD大鼠60只,随机分为2组,正常组和造模组;造模组采用二硝基苯磺酸(TNBS)/乙醇法复制UC大鼠模型;待复制模型成功后将造模组随机分为5组,分别为模型组、柳氮磺吡啶组(0.3 g·kg~(-1))以及健脾益肠散高、中、低剂量组(204,136,68 g·kg~(-1)),每组10只;灌胃相应药物21 d后,观察各组大鼠的一般状态和结肠黏膜组织损伤情况,免疫组化、蛋白免疫印迹法(Western blot)和实时荧光定量PCR(Real-time PCR)分别检测大鼠结肠组织中HSP70的蛋白和mRNA表达。结果:与正常组比较,模型组大鼠结肠黏膜损伤评分显著升高(P0.01),HSP70蛋白和mRNA表达均显著降低(P0.01)。与模型组比较,各给药组结肠黏膜损伤评分均显著降低(P0.01),各给药组均可增加结肠组织HSP70的蛋白和mRNA表达(P0.05,P0.01),其中以健脾益肠散高剂量组最为明显(P0.05,P0.01)。结论:健脾益肠散可能通过促进HSP70的表达而达到对UC大鼠结肠黏膜的免疫保护,从而发挥治疗作用。     

羟基磷灰石涂层热处理晶化工艺研究

目的：通过热处理方法提高羟基磷灰石（HA）涂层的结晶度、使脱落的羟基得到恢复。方法：通过等离子喷涂和热处理试验，利用X射线衍射（XRD）和红外吸收光普分析等手段研究了热处理对羟基磷石涂层相组成的影响。结果：热处理可使涂层中的非晶相发生晶化，并使羟基得到恢复；随着热处理温度的提高，涂层的结晶度提高，α－TCP等热分解相逐步转变为羟基磷灰石。保温时间达到2小时后，延长保温时间并不能提高涂层的结晶工。结论：在大气环境下，涂层的最佳热处理工艺是在700℃下保温2小时。     

热压技术制作烤瓷嵌体的实验研究

比较常规法和热压法所制作的实验标本挠屈强度的差异及两种方法所制作嵌体密合度的差异。采用双轴向三点支撑法测量实验标本的挠屈强度，用工具显微镜测量嵌体的密合度。结果表明，两种方法所制作的实验标本的挠屈强度无显著性差异（Ｐ＞０．０５）。用热压法制作的嵌体的密合度优于常规法（Ｐ＜０．０１）。热压技术设备简单、操作方便，可以提高瓷嵌体边缘密合度及外形准确性     

氟离子缓释正畸粘接剂缓释性能的研究

目的全面了解树脂型氟离子缓释正畸粘接剂(fluoride releasing adhesive，FRA)缓释氟离子的性能，建立一套评价FRA氟离子缓释性能的测试方法及指标。方法测定并计算FRA在一定时间中累计氟离子释放量，绘制出时间—氟离子累计释放量趋势线，并计算每单位质量FRA有效氟离子释放量。结果时间—氟离子累计释放量趋势线显示，FRA在90d内能持续、稳定地释放氟离子，每单位质量FRA在90d内有效氟离子释放量为449μg/g。结论本实验FRA试样具有稳定缓释氟离子的能力。     

大锥度牙胶尖热收缩性能的评价

目的观察4种不同品牌的0.06锥度的牙胶尖的热收缩性能。方法将4种牙胶尖末端固定在同一带刻度的平板上,尖端处于同一水平线,此水平线作为读数的零点,从30℃开始记录其长度变化;根据加热介质分为:A组,水浴加热;B组,甘油浴加热,采用Spss 13.0 for windows(English version)统计软件对结果进行相关分析。结果在50℃时4种不同品牌的牙胶尖都可以发生体积变化。牙胶尖的长度变化和温度具有明显的相关性。各牙胶尖之间的差别无统计学意义。不同的加热介质所出现不同的尺寸变化有统计学意义。结论4种不同品牌的牙胶尖分别在蒸馏水和甘油中加热,在50℃以上出现发生不同的热收缩,其在不同介质中发生变化不尽相同,提示在临床中不同根管封闭剂时可能发生不同的热收缩。     

Recognition and phagocytosis of multiple periodontopathogenic bacteria by anti-Porphyromonas gingivalis heat-shock protein 60 antisera

The present study has been performed to evaluate Porphyromonas gingivalis heat shock protein (HSP) 60 as a candidate vaccine to protect against multiple putative periodontopathic bacteria. Mouse anti-P. gingivalis HSP antisera demonstrated the elevated IgG antibody titers against the multiple bacteria tested and cross-reacted with heat-induced bacterial proteins of the target bacteria. The antisera also demonstrated a significantly higher opsonophagocytosis function against all the target bacteria than the control sera (P<0.01). We concluded that P. gingivalis HSP 60 could potentially be developed as a vaccine against multiple periodontopathic bacteria.     

Detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with Behcet's disease

We isolated bacteria from periodontal sites and mixed saliva in eight patients with Behcet's disease and surveyed them to determine whether they produced heat shock proteins (HSPs) and superantigen. Cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. Gram-negative anaerobic rods such as Prevotella intermedia, Fusobacterium nucleatum, and Capnocytophaga species were predominant in the isolates from the subgingival plaque samples. The Streptococcus mitis group was the most common type isolated from the saliva samples. To detect the production of HSPs, Western blot analyses were performed using a polyclonal rabbit antibody to Escherichia coli DnaK and a monoclonal antibody to Helicobacter pylori Gro-EL. Sonic extracts of 27 of the strains (79.4%) reacted with the antibody against E. coli DnaK. Nine of these 34 strains (26.5%) were found to produce HSPs that reacted with antibody to H. pylori Gro-EL. A total of 54 isolates were examined for superantigen activity against human peripheral leukocytes. Twenty-five gram-negative clinical strains isolated from chronic periodontitis lesions and 20 ATCC strains of microorganisms were also examined. We could not detect any superantigen activity in 500x diluted supernatant of the strains isolated from the eight patients with Behcet's disease. The present study indicates that the anaerobic strains isolated from the oral cavity of these patients produce HSPs, the production being related to Bechet's disease.     

热休克蛋白70在两个年龄组前磨牙成牙本质细胞表达的比较研究

目的观察热休克蛋白(HSP)70在前磨牙成牙本质细胞表达的增龄变化特点。方法采用免疫组化法结合图像分析系统,对HSP70在年老组(71~80岁)和年轻组(13~20岁)前磨牙成牙本质细胞和成牙本质细胞胞质中的表达强度进行比较。结果年老组成牙本质细胞内总的HSP70免疫组化染色的平均吸光度值比年轻组降低,而胞质内HSP70免疫组化染色的平均吸光度值平均值比年轻组略高,但无显著性差异。结论年老患者成牙本质细胞抵御急性刺激的能力下降可能与胞核内HSP70表达强度下降有关。     

氟化聚甲基丙烯酸甲酯体外释氟率的测定

目的:通过对含氟聚甲基丙烯酸甲酯体外释氟率的测定,为寻找较为理想的氟含量预防义齿基牙龋提供理论依据。方法:将分别含氟化钙、单氟磷酸钠10%、15%、20%、25%(重量比)5种不同浓度的热凝和自凝树脂各3块,分别于100ml、200ml去离子水中浸泡,定期取液,测量溶液中的氟离子浓度。结果:各浓度的含氟树脂块均能释放氟离子。结论:含单氟磷酸钠15%热凝树脂和10%的自凝树脂是比较好的含氟比例。含氟化钙20%热凝树脂和15%的自凝树脂是比较好的含氟比例。